卵巢癌中circ_0025033的表达及临床意义
Expression of circ_0025033 in ovarian cancer and its clinical significance
蒋丽 1黄丽萍 1唐利敏 1黄梦婷 2岳珍妮 3吴在归 4杨琪 4邱彩虹1
作者信息
- 1. 541001 广西桂林 桂林医学院附属医院妇科
- 2. 541001 桂林医学院附属医院超声科
- 3. 541199 桂林医学院第二附属医院妇科
- 4. 541001 桂林医学院
- 折叠
摘要
目的 探讨血浆环状RNA 0025033(circ_0025033)在卵巢癌诊断及治疗反应预测中的价值.方法 收集2020 年 9 月至 2022 年 6 月 165 例卵巢肿瘤患者作为研究对象,包括卵巢上皮性癌(EOC)100 例、卵巢良性肿瘤 30 例、卵巢交界性肿瘤 35 例.另选取同期 30 例健康体检者作为对照组.采集静脉血检测circ_0025033 的表达水平.采用受试者工作特征(ROC)曲线评估circ_0025033 表达诊断EOC的效能.58 例行单纯化疗的EOC患者根据治疗反应分为化疗敏感组(n=32)和化疗耐药组(n=26).分析治疗前血浆circ_0025033 水平预测EOC治疗反应的价值.结果 EOC患者血浆circ_0025033 水平显著高于健康体检者、卵巢交界性肿瘤患者和卵巢良性肿瘤患者(P<0.05);以健康志愿者、良性肿瘤、交界性肿瘤分别作为对照时,血浆circ_0025033 诊断 EOC 的曲线下面积(AUC)分别为 0.781(95%CI:0.698～0.864)、0.679(95%CI:0.579～0.779)和 0.731(95%CI:0.636～0.826).化疗前患者circ_0025033 水平与FIGO分期和淋巴结转移有关(P<0.05);化疗后患者circ_0025033 水平与FIGO分期和腹水有关(P<0.05).ROC曲线分析显示,最佳截断值为 2.685 时,治疗前血浆 circ_0025033 预测EOC患者化疗敏感性的 AUC 为 0.941(95%CI:0.884～0.998,P<0.001),灵敏度和特异度分别为 0.846 和0.937.结论 治疗前血浆circ_0025033 可能成为诊断EOC和预测EOC化疗疗效的生物标志物.
Abstract
Objective To investigate the value of plasma cyclic RNA 0025033(circ_0025033)in diagnosis and treatment response prediction of ovarian cancer.Methods One hundred and sixty-five patients with ovarian tumor from September 2020 to June 2022 were collected as the study objects,including 100 cases of epithelial ovarian cancer(EOC),30 cases of benign ovarian tumor and 35 cases of borderline tumor.Thirty healthy volunteers during the same period were selected as the control group.The expression of circ_0025033 was detected in venous blood.Receiver operating characteristic(ROC)curve was used to evaluate the efficacy of circ_0025033 expression in diagnosing EOC.According to the treatment response,58 patients with EOC were divided into chemotherapy sensitive group(n=32)and chemotherapy resistant group(n=26).The value of plasma circ_0025033 in predicting EOC chemotherapy response before treatment was analyzed.Results The plasma circ_0025033 level of EOC patients was significantly higher than that of healthy subjects,borderline tumors and benign ovarian tumors(P<0.05).The area under the curve(AUC)of plasma circ_0025033 for diagnosing EOC in healthy volunteers,benign tumors and borderline tumors were 0.781(95%CI:0.698-0.864),0.679(95%CI:0.579-0.779)and 0.731(95%CI:0.636-0.826),respectively.FIGO stage and lymph node metastasis were correlated with circ_0025033 level before chemotherapy(P<0.05).The circ_0025033 level was correlated with FIGO stage and ascites after chemotherapy(P<0.05).ROC curve results showed that the optimal cut-off value was 2.685.AUC of plasma circ_0025033 before treatment to predict chemotherapy sensitivity of EOC patients was 0.941(95%CI:0.884-0.998,P<0.001),and sensitivity and specificity were 0.846 and 0.937,respectively.Conclusion Plasma circ_0025033 may be a biomarker for the diagnosis of EOC and the prediction of chemotherapy efficacy before treatment.
关键词
卵巢癌/环状RNA/Circ_0025033/诊断/化疗敏感性Key words
Ovarian cancer/Circular RNA/Circ_0025033/Diagnosis/Chemotherapy sensitivity引用本文复制引用
出版年
2024
NETL
NSTL
万方数据