Expression and clinical significance of fibroblast growth factor receptor 2 in intrahepatic cholangiocarcinoma
Objective To investigate the expression and clinical significance of fibroblast growth factor receptor(FGFR)2 in intrahepatic cholangiocarcinoma(iCCA)tissues.Methods Two hundred and one iCCA formalin fixed paraffin embedded(FFPE)specimens that underwent liver resection at the 962 Hospital of the Joint Logistics Support Force from January 2018 to December 2022 were collected.In addition,13 cases of intrahepatic bile duct dilation(IHBD)and 34 cases of biliary epithelial neoplasia[23 cases of low-grade biliary epithelial neoplasia(BilIN-1/2)and 11 cases of high-grade biliary epithelial neoplasia(BilIN-3)]tissues were collected to detect FGFR2 expression in each group.The efficacy of FGFR2 expression in diagnosing iCCA was analyzed using receiver operating characteristic curve(ROC)analysis.Kaplan-meier method was used to plot the recurrence time(TTR)and overall survival time(OS)curves,and survival differences were analyzed using Log rank test.Using a multiple factor Cox proportional hazards regression model to analyze the influencing factors of TTR in iCCA patients.Results The HScore of FGFR2 expression in iCCA,IHBD,BilIN-1/2,and BilIN-3 tissues were 8.51(1.44,38.40),0(0,7.82),1.16(0,4.0),and 1.32(0,4.43),respectively.The FGFR2 expression level in the iCCA group was significantly higher than that in the other three groups(P<0.05).The ROC curve shows that the expression level of FGFR2 in tissues has good diagnostic efficacy for iCCA(P<0.05),and the area under the curve(AUC)is higher than 0.7.Multivariate Cox proportional hazards regression model analysis found that tumor diameter,FGFR2 expression,and TNM staging were independent prognostic factors affecting TTR in iCCA patients(P<0.05).Kaplan-Meier method showed that patients with high FGFR2 expression had shorter postoperative median TTR(5.60 months vs.9.90 months,P<0.001)and median OS(18.00 months vs.19.10 months,P=0.048)than the patients with low FGFR2 expression.Conclusion FGFR2 is helpful in distinguishing benign biliary diseases from iCCA,and high expression of FGFR2 suggests poor prognosis in iCCA patients.
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