Sophoroside and genistein are the major chemical constituents of the traditional Chinese medicine Sopho-ra japonica,in which they are abundant and possess a variety of biological active properties.Through reviewing ex-tensive domestic and international literature as well as relevant books,Sophoricoside and genistein were revealed to be of importance in a variety of applications,such as anticancer and cancer prevention,anti-osteoporosis,anti-car-diovascular disease,antioxidant,anti-inflammatory,contraceptive,neuroprotective,anti-radiation,immune-en-hancing,and anti-asthmatic,which could contribute to the development of traditional Chinese medicine and provide a broader perspective.