EBS and SHL are plant-specific chromatin reader proteins,which regulate target gene expression and related biological processes by specifically recognizing and binding to two antagonistic histone modifications.However,there have been no reports on the regulation of their protein functions by post-translational modifications.In this study,after the E.coli SUMOylation reconstruction system is used,it is found that both EBS and SHL have SUMOylation,and there is only one SU-MOylation band,no matter which SUMO molecular subtype is used as the donor.According to the difference of molecular weight between the SUMOylation band and the background protein band,it is inferred that both EBS and SHL are single-site single SUMOylation.Further site-directed mutagenesis experiments confirm that K216 is the single critical SUMOylation site for EBS,while the SUMOylation site for SHL remains to be further explored,but at least K111,K178,and K220 can be ex-cluded.This study lays a foundation for further exploration of the regulation of EBS and SHL protein functions by SU-MOylation modification.
维普
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