Papain is widely used in fields such as medicine,food,and daily chemicals.The improvement of papain activity and stability is beneficial to relax the restrictions of papain in industrial applications and improve economic benefits.In this paper,1,4-butanediol/choline chloride deep eutectic solvent was prepared,and its influence on papain activity and stability was studied.The results show that 1,4-butanediol/choline chloride deep eutectic solvent can enhance the activity of papain by increasing the mass transfer rate of substrates and enhancing the structural flexibility near enzyme active sites.The possi-ble interaction sites of 1,4-butanediol/choline chloride on papain were analyzed by molecular docking,and circular dichro-ism spectroscopy showed that the interaction did not cause significant changes in the secondary structure of papain.It is worth mentioning that storing papain in 1,4-butanediol/choline chloride deep eutectic solvent can significantly improve its stability.When papain dissolved in the 1,4-butanediol/choline chloride eutectic solvent system is stored at 37 ℃ or 4℃ for 8 days,its enzyme activity remains at 72.9％and 86.1％of the initial enzyme activity respectively.
维普
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