Screen-Testing of 150 Drugs and Toxic Compounds in Human Whole Blood by UPLC-TOF/MSE
UPLC-TOF/MSE was applied to the screen-testing of 150 drugs and toxic compounds in human whole blood.The human whole blood sample was prepared by liquid-liquid extraction,then separated on ACQUITY UPLC HSS C18 column.Mixtures of 5 rnmol · L-1 ammonium formate solution (pH 3.0) and 1％ (φ) formic acidacetonitrile solution mixed in different ratios were used as mobile phase in gradient elution.Modes of positive electric spray ionization and full-scanning were adopted in MS.Detection limits (3S/N) of the 150 drugs and toxic compounds found were in the range of 0.2-25μg · L-1.Matrix effects for 67 compounds were found in the range of 17.4％ to 126％,and values of recovery of liquid-liquid extraction were ranged from 29.7％ to 114％.
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万方数据
维普
