首页|基于化学激活荧光素酶表达基因法和标准方法评估动物源性固态食品中17种二噁英类化合物毒性当量的相关性比较

基于化学激活荧光素酶表达基因法和标准方法评估动物源性固态食品中17种二噁英类化合物毒性当量的相关性比较

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为使化学激活荧光素酶表达基因法(CALUX)在动物源性固态食品中二噁英类化合物毒性当量筛查中具有更好的适用性,使其能够与标准方法高分辨气相色谱-高分辨质谱法(HRGC-HRGC)的评估结果相互转换,分别基于CALUX和HRGC-HRGC评估了肉类、海鲜类和配方乳粉这3类典型动物源性固态食品中7种2,3,7,8-取代的多氯代二苯并二噁英(PCDDs)和10种2,3,7,8-取代的多氯代二苯并呋喃(PCDFs)的毒性当量,分析两种方法测定结果的相关性,并确定换算关系和适宜样品类型.结果表明:基于 HRGC-HRMS得到的样品中17种目标物的毒性当量(TEQ)下限值为0.001～0.106 pg·g-1,TEQ上限值为0.004～0.242 pg·g-1,远低于欧盟法规EC 1881/2006规定的限量值(3.5 pg·g-1);基于CALUX得到的肉类样品中17种目标物的毒性当量(BEQ)值为0.74～1.30 pg·g-1,海鲜类样品中17种目标物的BEQ值为0.31～0.63 pg·g-1,配方乳粉样品中17种目标物的BEQ值为0.043～0.074 pg·g-1;肉类和配方乳粉样品的BEQ值与TEQ值的相关性较差,而海鲜类样品的BEQ值与TEQ上限值相关性较好.
Correlation Comparison of Toxic Equivalents of 17 Dioxins in Solid Foods of Animal Origin Based on Chemically Activated Luciferase Expression Gene Method and Standard Method
In order to improve the applicability of chemically activated luciferase expression gene method(CALUX)in screening for toxic equivalents of dioxins in solid foods of animal origin,and to make it interconvertible with the evaluation results of the standard method high resolution gas chromatography-high resolution mass spectrometry(HRGC-HRGC),the toxic equivalents of 7 kinds of 2,3,7,8-substituted polychlorinated dibenzo-dioxins(PCDDs)and 10 kinds of 2,3,7,8-substituted polychlorinated dibenzo-furans(PCDFs)in three kinds of typical solid foods of animal origin including meat,seafood and formula milk based on CALUX and HRGC-HRGC were evaluated,and the correlation between the determined results of two methods was analyzed,and the conversion relationship and suitable sample type were determined.It was shown that the toxic equivalent(TEQ)lower limits of 17 targets in the samples based on HRGC-HRMS ranged from 0.001 to 0.106 pg·g-1,and the TEQ upper limits ranged from 0.004 to 0.242 pg·g-1,that were much lower than the limits of 3.5 pg·g-1 stipulated in European Union regulation EC 1881/2006.The toxic equivalent(BEQ)values of 17 targets in meat,seafood,and formula milk powder samples based on CALUX were in the ranges of 0.74-1.30,0.31-0.63 pg·g-1,and 0.043-0.074 pg·g-1,respectively.The correlation between BEQ and TEQ values of meat and formula milk samples was poor,while the correlation between BEQ and TEQ upper limit values of seafood samples was good.

chemically activated luciferase expression gene method(CALUX)high resolution gas chromatography-high resolution mass spectrometry(HRGC-HRMS)solid food of animal origintoxic equivalentcorrelation

王燕、张敏、朱晓艳、江玲丽、王如坤、钟莺莺、谭曜、保琦蓓

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宁波海关技术中心,宁波 315012

南京海关危险货物与包装检测中心,常州 213000

宁波卫生职业技术学院,宁波 315100

化学激活荧光素酶表达基因法(CALUX) 高分辨气相色谱-高分辨质谱法(HRGC-HRGC) 动物源性固态食品 毒性当量 相关性

浙江省教育厅科研项目宁波市自然科学基金宁波市自然科学基金宁波市公益类科技计划重点项目宁波卫生职业技术学院校级科研项目宁波中盛公司科技项目

Y202045000202003N41892023J1872022S0062020Z062021ZS011

2024

10.11973/lhjy-hx202401001

理化检验-化学分册
上海材料研究所

理化检验-化学分册

CSTPCD北大核心
影响因子：0.647
ISSN：1001-4020
年,卷(期)：2024.60(1)
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