Detection of Lung Cancer Marker CYFRA21-1 in Serum by Electrochemical Immunosensor Modified with Nanocomposite
An electrochemical immunosensor modified with nanocomposite was constructed to detect cytokeratin 19 fragment antigen 21-1(CYFRA21-1)which was a lung cancer marker.Ordered mesoporous carbon CMK-3 was combined with carboxylated multi walled carbon nanotubes(CMWCNTs)to improve the electron transfer rate and achieve electrochemical signal amplification.Au nanoparticles(AuNPs)were combined furtherly to prepare an electrochemical immunosensor on a glassy carbon electrode.AuNPs could be uniformly compounded with CMK-3@CMWCNTs and combined with the CYFRA21-1 antibody through the Au-S bond,providing a large number of biological binding sites for the antibody and improving the sensitivity of the sensor,so as to realize the detection of CYFRA21-1 in serum through antigen-antibody specific reaction.Scanning electron microscope characterization showed that AuNPs were embedded in the surface and pores of CMK-3@CMWCNTs.The trends of cyclic voltammetry(CV)curves showed that the constructed electrochemical immunosensor was sensitive and effective.The linear range of the constructed electrochemical immunosensor for detection of CYFRA21-1 in serum was 0.5-1×104 ng·L-1,with detection limit(3S/N)of 0.2 ng·L-1.The spiked recovery of CYFRA21-1 in the serum sample was in the range of 91.4%-102%,with RSDs(n=6)of the determined values less than 7.0%.
electrochemical immunosensorcytokeratin 19 fragment antigen 21-1(CYFRA21-1)Au nanoparticleordered mesoporous carbon
万方数据
维普
