Human umbilical cord mesenchymal stem cells for premature ovarian insufficiency:a systematic review
Objective To investigate the effect of human umbilical cord mesenchymal stem cells(hUC-MSCs)on the treatment of pre-mature ovarian insufficiency(POI)in mice.Methods A computer was used to search the major Chinese and English medical databas-es,and the mouse animal studies from the establishment of the database to June 2023 were searched,and then the references included in the research were searched manually to further identify other experimental studies that met the conditions.Two researchers independ-ently searched,screened and evaluated medical databases based on search terms,and used the SYRCLE bias risk tool to assess the risk of bias in animal studies.All statistical analyses were performed using Review Manager 5.4 software.Results A total of 9 studies were included.In the POI mouse model,compared with the control group,the use of hUC-MSCs in the experimental group increased se-rum estrogen levels(mean difference = 22.05,95% confidence interval 7.37-36.74,P<0.01)and anti-Mullerite hormone levels(mean difference =166.12,95% confidence interval 48.29-283.95,P<0.01),decreased the level of serum follicle-stimulating hor-mone(mean difference =-2.77,95% confidence interval-4.45--1.10,P<0.01),increased the number of primary follicles(mean difference =11.02,95% confidence interval 10.56-11.47,P<0.01)and the number of secondary follicles(mean difference = 7.49,95% confidence interval 1.90-13.08,P<0.01),decreased the number of atretic follicles(mean difference =-7.67,95% con-fidence interval-13.94--1.41,P =0.02),increased the body mass of mice after treatment(mean difference =3.92,95% confi-dence interval 0.51-7.34,P =0.02)and ovarian mass(mean difference =1.06,95% confidence interval 0.88-1.23,P<0.01),short-ened the estrus cycle of mice(mean difference =-1.41,95% confidence interval-1.97--0.85,P<0.01),increased number of lit-ter born(mean difference =6.18,95% confidence interval 5.24-7.12,P<0.01),but the level of serum luteinizing hormone(mean difference =0.16,95% confidence interval-1.99-2.31,P =0.88)and the number of original follicles after treatment(mean differ-ence =9.82,95% confidence interval-4.21-23.85,P =0.17)had no significant improvement.Conclusion Stem cell therapy is ef-fective in POI mouse models,and it is a potential therapeutic strategy to restore fertility in POI patients.
Human umbilical cord mesenchymal stem cellsPremature ovarian insufficiencySystematic evaluationHor-mones
NETL
NSTL
万方数据
维普
