32P不同温度不同照射剂量对SD大鼠放射性皮肤损伤初步研究
Preliminary study on radiation skin injury of SD rats at different temperature and dose of 32 P
郭佳 1程凤 2曹滨验 3游平飞 4王秋1
作者信息
- 1. 北部战区总医院 核医学科,辽宁 沈阳 110016
- 2. 北部战区总医院 急诊医学科,辽宁 沈阳 110016
- 3. 大连医科大学,辽宁 大连 116000
- 4. 中国医科大学,辽宁 沈阳 110000
- 折叠
摘要
目的 探讨32P不同温度不同照射剂量造成SD大鼠放射性皮肤损伤的病理状态.方法 选取 48 只 6～8 周雄性SD清洁级大鼠为研究对象.采用随机数字表法将48 只大鼠随机分为A组(室温下10 Gy)、B组(低温下10 Gy)、C组(室温下30 Gy)、D组(低温下30 Gy)、E组(室温下60 Gy)、F组(低温下 60 Gy),每组各 8 只.采用32P发射的β射线进行照射.计算大鼠皮肤损伤面积、进行皮肤损伤评分及病理切片苏木素伊红染色.比较各组大鼠不同照射时间皮肤损伤面积百分比、皮肤损伤评分及病理学变化.结果 F组大鼠不同照射时间的皮肤损伤面积百分比均高于E组,差异均有统计学意义(P<0.05).D组照射后1 周、照射后2 周的皮肤损伤评分均高于C组,差异均有统计学意义(P<0.05).低温中的B组、D组、F组大鼠受照皮肤的病理改变均早于室温下的A组、C组、E组,且损伤程度较重.结论 低温环境下的皮肤β射线损伤发生早、损伤进展快,且其损伤程度随照射剂量增加而加重.
Abstract
Objective To investigate the pathological status of radiation skin injury in SD rats caused by different temperature and dose of 32 P.Methods Forty-eight male SD clean rats were selected as the study subjects.A total of 48 rats were randomly divided into group A(10 Gy at room temperature),group B(10 Gy at low temperature),group C(30 Gy at room temperature),group D(30 Gy at low temperature),group E(60 Gy at room temperature)and group F(60 Gy at low temperature)with 8 rats in each group.It was irra-diated by 32 P beta rays.The skin injury area of rats was calculated,the skin injury score was performed and the pathological sections were stained with hematoxylin eosin.The percentage of skin damage area,skin damage score and pathological changes of rats in differ-ent irradiation time were compared.Results The percentage of skin damage area in group F was higher than that in group E at differ-ent irradiation times,and the differences were statistically significant(P<0.05).The skin injury scores of group D 1 week and 2 weeks after irradiation were higher than those of group C,and the differences were statistically significant(P<0.05).The pathological changes of exposed skin of rats in groups B,D and F at low temperature were earlier than those in groups A,C and E at room tempera-ture,and the damage degree was more serious.Conclusion The skin β-ray damage occurs early and progresses rapidly in low temper-ature environment,and the damage degree increases with the increase of irradiation dose.
关键词
照射剂量/放射性皮肤损伤/β射线/皮肤损伤评分Key words
Irradiation dose/Radiation skin damage/Beta rays/Skin injury score引用本文复制引用
基金项目
军队后勤科研重点课题(BLB19J012)
军队护理创新与培育专项(2021HL057)
出版年
2024
NETL
NSTL
万方数据