Correlation between phosphodiesterase 4B and doxorubicin-induced cardiotoxicity in mice
Objective To investigate the correlation between phosphodiesterase 4B(PDE4B)and doxorubicin-induced cardiotoxicity(DIC)in mice.Methods Twelve C57BL/6J mice were randomly divided into saline group and DIC group,with 6 mice in each group.In the DIC group,doxorubicin(DOX)was injected intraperitoneally to establish a mouse DIC model.On day 15 after DOX injection,the cardiac function of mice in saline group and DIC group was evaluated by ultrasound.Real-time quantitative reverse tran-scription polymerase chain reaction(PCR)and Western blot were used to detect PDE4B mRNA and PDE4B protein levels in saline group and DIC group.After DOX stimulation of HL-1 cardiomyocytes for 24 h,real-time quantitative reverse transcription PCR and Western Blot were used to detect PDE4B mRNA and PDE4B protein levels in control group(not given DOX stimulation)and DOX group(given DOX stimulation).Results Compared with saline group,left ventricular ejection fraction and short axis shortening rate in DIC group were significantly decreased(P<0.05).Compared with saline group,the mRNA and protein levels of PDE4B in my-ocardial tissue of DIC group were significantly increased(P<0.05).In cardiomyocytes,compared with control group,the mRNA and protein levels of PDE4B of DOX group were significantly increased(P<0.05).Conclusion The expression of PDE4B is increased in myocardial tissue and cardiomyocytes after DOX stimulation.
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