Pathogenic mechanism of radiation-induced acute lung injury based on proteomics
Objective To investigate the pathogenic mechanism of radiation-induced acute lung injury in rats based on proteomics.Methods Twenty healthy male SD rats with body weight of 180-220 grams were randomly divided into control group and radiation group(20 Gy lung irradiation of rats),with 10 rats in each group.The animal model of lung injury was established by linear accelerator irradiation of 20 Gy.After the rats were killed,whole lung tissue samples were taken,proteins were extracted,and proteomic detection was carried out based on liquid chromatography tandem mass spectrometry.The differential proteins after radiation lung injury were an-alyzed through biogenic analysis by DAVID,and meaningful pathways were screened according to the change characteristics of the o-verall inflammatory proteins after radiation lung injury.Results Through protein quantitative screening,200 differentially expressed proteins were screened out between the radiation group and the control group.According to the results of enrichment analysis of inflam-mation-related proteins,the most significant 9 pathways were screened out,including the regulation of the inflammatory response,posi-tive regulation of transforming growth factor-β receptor signaling pathway,mucosal cells of the innate immune response,interleukin-7 reaction,positive regulation of interleukin-8 production,positive regulation of NIK/NF-kappaB signals,positive regulation of neutrophil chemotaxis,neutrophil chemotaxis,activation of T cells by antigenic contact with T cell receptors bound to MHC molecules on antigen-presenting cells.Conclusion Radiation lung injury may be closely related to inflammatory response,antioxidant stress and expression of inflammatory regulatory proteins.
Radiation lung injuryProteomicsInflammatory responseLiquid chromatography tandem mass spectrometry
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