Effect of ultrasonic micro-arc oxidation and polylacticcoglycollic acid composite coating porous magnesium materials on re-pairing jaw defect model in large rabbits
Objective To investigate the effect of ultrasonic micro-arc oxidation(MAO)and polylacticcoglycollic acid(PLGA)compos-ite coating porous magnesium materials on repairing jaw defect model in large rabbits.Methods A total of 18 healthy male New Zeal-and white rabbits were randomly divided into 3 groups with 6 rabbits in each group.Porous magnesium cylindrical material was soaked in ultrasonic MAO and PLGA solution to obtain a new type of porous magnesium material.The new porous magnesium material(Mg-MAO-PLGA group)and the traditional MAO porous magnesium column(Mg-MAO group)were implanted into the jaw bone defect area of large rabbits,and the jaw bone defect self-healing group(control group)was set up.After 2,4 and 8 weeks,the material changes and the healing of bone defect were analyzed by scanning electron microscopy,energy spectrum analysis,hematoxylin-eosin(HE)staining and bone morphogenetic protein(BMP)-2 immunohistochemistry.Results The surface of Mg-MAO group formed a uniform tube array structure,while the pore structure of Mg-MAO-PLGA group was covered by coating.After surgery,cone-beam CT showed that the im-plant was in good position.The interface of the Mg-MAO-PLGA group was clear and continuous,and the boundary area was smaller than that of the Mg-MAO group.The Ca and P signals in the boundary region of Mg-MAO-PLGA group were stronger than those in Mg-MAO group at the same time point,that was,new bone formation was more active.The density of new bone tissue in Mg-MAO-PL-GA group was higher,and the positive expression rate of BMP-2 was higher than that in Mg-MAO group and control group.Conclusion The porous magnesium material treated with ultrasonic MAO and PLGA composite coating can effectively slow down the reaction rate in vivo and contribute to the formation of new bone in the bone defect area.This material has potential application value in promoting the healing of bone defects and is expected to provide a new idea for the healing treatment of bone defects.
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