The nitrogen removal performance of the filler was investigated by using natural zeolite as the main material to prepare a modified porous zeolite hybrid filler,which simulated ammonia nitrogen wastewater and aeration biofilter technology(BAF)after the maturation of the packed column hanging film,and was operated in three stages of ion exchange adsorption,microbial regeneration and drenching,while the microbial characteristics of the packed biofilm were characterized by PCR-DGGE(denaturing gradient gel electrophoresis)electrophoresis.The results showed that when the hydraulic residence time of ion exchange adsorption stage was 3.0 h,the best comprehensive ammonia nitrogen removal efficiency(82.3%-94.7%)and high nitrogen recovery(88.46%)were obtained from the packing;the microbial community richness was more in the outer layer of the packing than in the inner layer.Moreover,the upper and lower layers of the system were more diverse than their middle counterparts.The clustering results of different strips were consistent with the similarity of the strips and the real situation.Meanwhile,the genus of bacteria(hydrogenophilic bacteria)and nitrifying and denitrifying bacteria that can degrade various organic pollutants were detected in the strips.