The environmental protection self-assessment work of a natural science laboratory construction project is taken as an example to introduce the main process,key points of self inspection,and monitoring plan of environmental protection self-assessment for laboratory construction projects.It focused on analyzing the main issues that should be paid attention to in the self-assessment work of environmental protection related laws,rules and regulations,and laboratory pollutant emissions,analyzed the characteristics of environmental protection self-assessment work in natural science laboratories and proposed corresponding strategies,in order to provide reference for environmental protection self-assessment of similar projects after completion.