茜草根茎中蒽醌的提取工艺研究
Study on Extraction Process of Anthraquinone From Rhizomes of Rubia
曹丽丽 1阮长青 1高金玲 1李雪 1赵觅 1李强2
作者信息
- 1. 黑龙江八一农垦大学理学院,黑龙江 大庆 163319
- 2. 中国石油大学(北京)克拉玛依校区,新疆 克拉玛依 834000
- 折叠
摘要
以乙醇为溶剂,采用超声波辅助法提取茜草根茎中的蒽醌类化合物.通过超声时间、溶剂用量和溶剂浓度等单因素试验探寻蒽醌的提取条件,并利用三因素三水平的 Box-Behnken 响应面分析法试验设计优化提取工艺,得到最佳提取条件为超声时间 31.29 min、溶剂用量 13.47 mL、溶剂浓度81.15%,茜草根茎中蒽醌提取率的理论预测值为7.64%.验证试验结果表明,此条件下茜草根茎中蒽醌提取率为7.62%,与理论预测值较为接近.因此,响应面分析法可得到最优的提取工艺.最后通过紫外和红外光谱法对其结构进行了表征.
Abstract
The anthraquinones in the rhizome of Rubia were extracted by ultrasonic assisted method using ethanol as the solvent.The extraction conditions of anthraquinone were explored by single-factor tests such as sonication time,solvent dosage and solvent concentration.A Box-Behnken response surface analysis test based on single-factor experiments of three factors at three levels was applied to optimize the extraction process.The optimum conditions were as follows:ultrasonic time 31.29 min,solvent dosage 13.47 mL,solvent volume fraction 81.15%.The highest theoretical prediction extraction rate of anthraquinone in the rhizomes of Rubia was 7.64%.The verification experiments under these conditions showed that the extraction rate of anthraquinone in the rhizomes of Rubia was 7.62%,which was close to the theoretical prediction.Therefore,the optimal extraction process can be obtained by the method of response surface analysis.Finally,the structure was characterized by UV and IR spectroscopy.
关键词
茜草根茎/蒽醌/超声波/响应面法Key words
Rhizomes of Rubia/Anthraquinone/Ultrasonic/Response surface method引用本文复制引用
基金项目
大庆市指导性科技计划(zd-2023-45)
出版年
2024
NETL
NSTL
万方数据