Traditional biochemical methods for enzyme detection are mainly based on antibody-based immunoassays,which lack the ability to monitor the spatial and temporal distribution of enzymes in living cells and in vivo,especially the REDOX activity of enzymes.In this article,a comprehensive summary of the advances made in recent years in the development of small molecule and material-based fluorescent probes for sensitive detection of the activity of enzymes associated with many human diseases was presented.
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