摘要
以油橄榄果渣熊果酸为材料,以沙门氏菌为受试菌,通过生长曲线、培养液电导率、核酸含量、细胞内腺苷三磷酸(adenosine triphosphate,ATP)含量的变化以及细菌电镜扫描方法,探讨熊果酸对沙门氏菌的抑菌机理.结果表明,经油橄榄果渣熊果酸处理后的沙门氏菌细胞膜受到破坏,其生长速率在各时间段受到抑制,菌液电导率、核酸含量均显著增加,ATP含量降低.电镜扫描结果表明熊果酸使菌体皱缩内陷,出现孔洞,破坏细胞膜完整性,造成细胞内物质泄漏.说明油橄榄果渣熊果酸能抑制沙门氏菌的生长,作用机制是改变了沙门氏菌细胞膜的通透性,并破坏了菌体细胞的形态结构.
Abstract
Using olive pomace ursolic acid as the material and Salmonella as the test organism,the growth curve,culture fluid conductivity,nucleic acid content,intracellular adenosine triphosphate(ATP)content changes and bacterial electron microscopy scanning methods to explore the inhibition mechanism of ursolic acid on Salmonella.The results showed that the cell membranes of Salmonella spp.treated with ursolic acid from olive pomace were disrupted and their growth rate was inhibited at all times,and the conductivity and nucleic acid content of the culture broth increased significantly and the ATP content decreased.Electron microscopy scans showed that ursolic acid caused the bacterium to crumple and sink inward,developing holes and disrup-ting the integrity of the cell membrane,causing leakage of intracellular material.This suggests that ursolic acid from olive pomace inhibits the growth of Salmonella and the mechanism of action is to alter the permeabili-ty of the Salmonella cell membrane and disrupt the morphological structure of the bacteriophage.
基金项目
甘肃省科技厅青年科技项目(21JR1RE297)
甘肃省教育厅创新基金(2023B-139)
甘肃省科技厅技术创新引导计划科技专员专项(23CXGE0009)
天水师范学院大学生创新创业训练计划(CX20220008)
国家科技期刊平台
NSTL
万方数据