非受体酪氨酸激酶2可能是银屑病的潜在治疗靶点
PTPN2 can be a potential target for the treatment of psoriasis
刘守刚 1刘芳华 2陈永锋1
作者信息
- 1. 南方医科大学皮肤病医院,广东 广州 510091
- 2. 广东省人民医院赣州医院,江西 赣州 341099
- 折叠
摘要
目的 探讨非受体酪氨酸激酶2(PTPN2)在银屑病中的表达及调控机制,分析PTPN2对银屑病相关性免疫的影响,以确定PTPN2是否可以作为银屑病的潜在治疗靶点.方法 通过R语言(3.6.3版本)绘制PTPN2在银屑病和对照组中的表达差异以及PTPN2的接受者操作特征曲线(ROC).基因富集分析软件(GSEA)展示PTPN2在银屑病数据集中的富集通路,蛋白质相互作用分析数据库(STRING)展示PTPN2的共表达基因的相互作用网络.CIBERSORT软件分析银屑病数据集的免疫细胞分布及表达情况.通过R语言(3.6.3版本)分析免疫反应通路和免疫反应基因集在银屑病中的差异表达及与PTPN2的相关性.结果 PTPN2 mRNA在银屑病皮损中高表达.银屑病皮损中免疫浸润细胞、免疫反应通路和免疫反应基因集均存在异常差异表达.PTPN2与银屑病中部分免疫浸润细胞、免疫反应通路和免疫反应基因集存在相关性.结论 PTPN2 mRNA在银屑病皮损中高表达,并可能通过调控免疫微环境参与银屑病的发生发展.
Abstract
Objective To investigate the expression and regulatory mechanism of PTPN2 in psoriasis,and to analyze the effects of PTPN2 on psoriasis related immunity in order to determine whether PTPN2 can be used as a potential therapeutic target for psoriasis.Methods R language(3.6.3 version)was used to determine the difference in expression levels of PTPN2 between pso-riatic patients and controls,and the receiver operating characteristic(ROC)curve of PTPN2.The Gene Enrichment Analysis software(GSEA)and the Protein Interaction Analysis Database(STRING)were used to determine enrichment pathway of PTPN2 in the psoriasis dataset,and the interaction network of PTPN2 co-expressed genes.The distribution and expression of immune cells in psoriasis datasets were analyzed with CIBERSORT software.The differential expression of im-mune response pathways and immune response gene sets in psoriasis and their correlation with PT-PN2 were analyzed using R language(version 3.6.3).Results PTPN2 mRNA was highly ex-pressed in psoriatic lesions.Immune infiltrating cells,immune response pathways,and immune response gene sets were abnormal in psoriatic lesions.PTPN2 was associated with some immune infiltrating cells,immune response pathways,and immune response gene sets in psoriasis.Con-clusions PTPN2 mRNA is highly expressed in psoriatic lesions,and may participate in the oc-currence and development of psoriasis by regulating the immune microenvironment.
关键词
PTPN2/免疫细胞浸润/免疫反应/银屑病Key words
PTPN2/immune cell infiltration/immune response/psoriasis引用本文复制引用
基金项目
广东省医学科学技术研究项目(B2019121)
出版年
2024
国家科技期刊平台
NSTL
万方数据