双波长脉冲染料激光抑制血管瘤内皮细胞增殖并促进其凋亡
Dual-wavelength pulsed dye laser inhibits proliferation and promotes apoptosis of hemangi-oma vascular endothelial cells
狄奇 1李三林 1张高磊 1陈程浩 1曹佳捷 1熊祎 1张靖 2王昊 3刘景 3申刚1
作者信息
- 1. 首都儿科研究所附属儿童医院,北京 100020
- 2. 广东省人民医院,广东 广州 519041
- 3. 郑州大学附属儿童医院,河南 郑州 450066
- 折叠
摘要
目的 探讨双波长脉冲染料激光对血管瘤内皮细胞(HemECs)的增殖和凋亡的影响.方法 体外分离培养HemECs并随机分为4组:脉冲染料激光组(PDL,595 nm波长)、Nd∶YAG激光(1 064 nm波长)组、双波长组(595 nm PDL+1 064 nm Nd∶YAG)、对照组(不照射激光).于照射结束后第1、3、7天,采用CCK-8法检测各组HemECs的增殖能力,流式细胞术检测凋亡率,Western blot检测新生血管生成相关蛋白VEGF、bFGF的表达水平.结果 激光照射后HemECs细胞较对照组细胞逐渐变长、变疏.照射后第1天,与对照组相比,双波长组HemECs的增殖抑制率显著升高(P=0.015),而PDL组、Nd∶YAG组的增殖抑制率均无明显改变(均P＞0.05);照射后第3、7天,与对照组相比,PDL组、Nd∶YAG组及双波长组的HemECs的增殖活性、VEGF和bFGF蛋白表达水平均显著降低(均P＜0.001),增殖抑制率与凋亡率均显著升高(均P＜0.001),且双波长组较PDL组、Nd∶YAG组更明显(均P＜0.05).结论 双波长脉冲染料激光可显著抑制HemECs增殖、促进其凋亡,降低血管生成因子水平,其作用较595 nm PDL或1 064 nm Nd∶YAG单波长激光照射更明显.
Abstract
Objective To investigate the effects of dual-wavelength pulsed dye laser on the proliferation and apoptosis of hemangioma vascular endothelial cells(HemECs).Methods The isolated HemECs were randomly divided into four groups:pulsed dye laser group(PDL,595 nm wavelength),Nd∶YAG laser(1 064 nm wavelength)group,dual-wavelength group(595 nm PDL+1 064 nm Nd∶YAG)and control group(no laser treatment).After 1,3,and 7 d of treat-ment,the proliferation and apoptosis rate of HemECs were detected using CCK-8 and flow cytome-try,respectively.The expression levels of angiogenesis related proteins,VEGF and bFGF,were detected with Western blotting.Results Compared with the control group,laser treatment made HemECs longer and sparser.After one day of treatment,the dual-wavelength laser significantly in-hibited HemEC proliferation(P=0.015).However,neither PDL nor Nd∶YAG significantly changed proliferation rate of HemECs(P＞0.05 vs.controls).After 3 and 7 days of treatment,the proliferation activity,expression levels of VEGF and bFGF proteins of HemECs were markedly decreased in the all laser-treated groups(P＜0.001 vs.controls),while the rates of proliferation inhibition and apoptosis were significantly increased(P＜0.001 vs.controls),with a more promi-nent effect in the dual-wavelength laser-treated group(P＜0.05 vs.PDL and Nd∶YAG groups).Conclusions Dual-wavelength pulsed dye laser can significantly inhibit the proliferation and pro-mote apoptosis of HemECs,in addition to reduction in expression levels of angiogenesis factors.The effects of dual-wavelength pulsed dye laser are superior to that of either 595 nm PDL or 1 064 nm Nd:YAG single wavelength laser.
关键词
血管瘤/血管瘤内皮细胞/脉冲染料激光/增殖/凋亡Key words
hemangioma/hemangioma endothelial cells/pulsed dye laser/proliferation/apoptosis引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据