Paeoniflorin improves psoriasis by modulating neutrophil extracellular traps in a mouse model of psoriasis
Objective To investigate the effects of paeoniflorin on imiquimod-induced psoria-sis in a mouse model and the underlying mechanisms.Methods Fifteen healthy BALB/C mice were randomly divided into the control,model,and model group-treated with low-,medium-,and high-doses of paeoniflorin,once daily for 7 days.The back skin of mice in the control and model groups were treated topically with Vaseline and imiquimod ointment,respectively.The imiquimod-treated mice were intragastrically given either saline or paeoniflorin at doses of 75 mg/(kg·d),150 mg/(kg·d)and 300 mg/(kg·d),twice daily.The erythema and scaling on the treated ar-ea of the skin were observed.The skin biopsies were obtained from the treated area for H&E stai-ning to observe the changes in histopathology and the skin thickness of the lesions,while immuno-fluorescence was applied to detect neutrophil infiltration.The expression levels of TNF-α and IL-17 mRNA were assessed with RT-PCR.In addition,Western blot was used to assess the expres-sion of citrullinated histone 3(CitH3).Results Compared with the model group,topical treat-ment with paeoniflorin attenuated multiple changes in the imiquimod-treated skin,including re-duced erythema and scaling,decreased epidermal thickness and neutrophil infiltration,down-reg-ulation of TNF-α and IL-17 mRNA expression(P＜0.001 vs.imiquimod alone for all)in addition to reduction in expression levels of CitH3(all P＜0.05 vs.imiquimod alone).Moreover,paeoni-florin-induced changes in expression levels of IL-17 and CitH3 were dose-dependent(P＜0.05 for both).Conclusion Paeoniflorin can modulate neutrophil extracellular traps,resulting in down-regulation of the expression levels of inflammatory cytokines,consequently alleviating cutaneous inflammation in a mouse model of psoriasis.
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