Metabolomic profiling of cerebrospinal fluid in neurosyphilis:identification of potential diag-nostic biomarkers
Objective This study aims to analyze metabolites in the cerebrospinal fluid(CSF)of neu-rosyphilis patients to identify potential diagnostic markers.Methods Untargeted metabolomics by liquid chro-matography-mass spectrometry was used to analyze metabolites in CSF samples from five neurosyphilis and five syphilis/non-neurosyphilis patients.After quality control and normalization,data analyses(principal compo-nent analysis,orthogonal partial least squares-discriminant analysis,hierarchical clustering)were conducted to identify differential metabolites.Results The content of metabolites(Pro-leu,nudifloramide,N-oleoyl gly-cine,DMP 777,(±)-propionylcarnitine,and 3β-hydroxy-5-cholenoic acid)in neurosyphilis patients was 1.7-to 4.5-fold of that in the non-neurosyphilis patients.Conclusions The metabolites,particularly nudiflo-ramide and N-oleoyl glycine,which have been implicated in other neurological disorders,warrant further inves-tigation into their roles in the pathogenesis of neurosyphilis.
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