神经梅毒脑脊液代谢组学分析:潜在诊断生物标记物的鉴定
Metabolomic profiling of cerebrospinal fluid in neurosyphilis:identification of potential diag-nostic biomarkers
贺媛媛 1吴嘉欣 2陈钊琪 2邓梓亮 1王海鹰 1柯吴坚2
作者信息
- 1. 南方医科大学公共卫生学院,广东 广州 510515
- 2. 南方医科大学皮肤病医院,广东 广州 510091
- 折叠
摘要
目的 分析神经梅毒患者的脑脊液代谢物,以确定潜在的诊断标志物.方法 利用液相色谱-质谱联用技术的非靶向代谢组学,分析5例神经梅毒患者和5例梅毒/非神经梅毒患者的脑脊液样本.将原始数据经过质量控制和归一化处理后,进行数据分析(主成分分析、正交偏最小二乘判别分析、层次聚类分析)确定两组间的差异代谢物.结果 神经梅毒患者体内,有6种代谢物显著升高,分别是 Pro-leu、nudifloramide、N-oleoyl glycine、DMP 777、(±)-propionylcarnitine 和 3 β-hydroxy-5-cholenoic acid.这6种代谢物两组间的差异倍数范围是1.7-4.5.结论 所发现的代谢物,特别是nudifloramide和N-oleoyl glycine,曾被报道与其他神经系统疾病有关,其在神经梅毒发病机制中的作用值得进一步研究.
Abstract
Objective This study aims to analyze metabolites in the cerebrospinal fluid(CSF)of neu-rosyphilis patients to identify potential diagnostic markers.Methods Untargeted metabolomics by liquid chro-matography-mass spectrometry was used to analyze metabolites in CSF samples from five neurosyphilis and five syphilis/non-neurosyphilis patients.After quality control and normalization,data analyses(principal compo-nent analysis,orthogonal partial least squares-discriminant analysis,hierarchical clustering)were conducted to identify differential metabolites.Results The content of metabolites(Pro-leu,nudifloramide,N-oleoyl gly-cine,DMP 777,(±)-propionylcarnitine,and 3β-hydroxy-5-cholenoic acid)in neurosyphilis patients was 1.7-to 4.5-fold of that in the non-neurosyphilis patients.Conclusions The metabolites,particularly nudiflo-ramide and N-oleoyl glycine,which have been implicated in other neurological disorders,warrant further inves-tigation into their roles in the pathogenesis of neurosyphilis.
关键词
神经梅毒/代谢组学分析/脑脊液/诊断生物标志物Key words
neurosyphilis/metabolomic profiling/cerebrospinal fluid/diagnostic biomarkers引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据