Extraction efficiency of fully automated nucleic acid extractor on different types of ASFV and PEDV samples
The study aimed to evaluate the extraction efficiency of a fully automated nucleic acid extractor on DNA and RNA nucleic acids using various types of positive samples for African swine fever virus(ASFV)and porcine epidemic diarrhea virus(PEDV).The objective was to assist animal disease detection laboratories in understanding how to assess whether a fully automated nucleic acid extractor meets their testing requirements.Positive samples of different ASFV types and PEDV-positive fecal swab samples were selected for the experiment.These samples were subjected to a 10-fold gradient dilution,and the detection results were compared with known original values.The results showed that the stability of the nucleic acid extractor used in the experiment met the requirements but exhibited poor sensitivity,potentially leading to false-negative results in samples with low virus content,such as those from the environment,personnel,or vehicles.In terms of interference resistance,the nucleic acid extractor produced false-negative results in the extraction of nucleic acids from throat swabs.The interference resistance of the instrument was unsatisfactory for anal swabs and environmental samples,which could lead to false-negative results.The instrument was not influenced by the sample type,resulting in false-positive results.
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