喀什地区某牛场环形泰勒虫的分子生物学鉴定及分析
Molecular biological identification and analysis of Thaleria annulata in a cattle farm in Kashgar
黄凯俊 1何波 2李飞 3熊娜 1胡静雷 1邵程祥 1武军元1
作者信息
- 1. 塔里木大学动物科学与技术学院,新疆阿拉尔 843300;新疆兵团塔里木动物疫病诊断与防控工程实验室,新疆阿拉尔8433003
- 2. 哈密市伊州区动物疫病预防控制中心,新疆哈密 839000
- 3. 阿克苏地区动物疫病控制诊断中心,新疆阿克苏 843000
- 折叠
摘要
试验旨在鉴定喀什地区某牛养殖场的西门塔尔牛的病因,对疑似感染泰勒虫的病牛血液进行PCR检测.运用PCR方法对泰勒虫18S rRNA基因进行扩增、测序,通过MEGA 7.0软件构建系统进化树,同时进行遗传进化分析.结果显示,18SrRNA基因序列与环形泰勒虫中国新疆分离株(OM438135.1)同源性为100.0％,表明该场病牛为环形泰勒虫感染;系统进化树分析结果显示,该虫株与中国和巴基斯坦分离株亲缘关系比较近.研究表明,导致喀什某牛场西门塔尔牛患病的病原体是环形泰勒虫.
Abstract
The purpose of the experiment was to identify the etiology of Simmental cattle in a cattle farm in Kashgar,and to detect the blood of cattle suspected to be infected with Thaleria by PCR.The 18S rRNA gene was amplified and sequenced by PCR,the phylogenetic tree was constructed by MEGA 7.0 software,and the genetic evolution was analyzed at the same time.The results showed that the sequence of 18S rRNA gene was 100.0％homologous to that of Theleria annulata isolated from Xinjiang,China(OM438135.1),indicated that the infected cattle were infected with Theleria annulata.Phylogenetic tree analysis showed that the strain was closely related to Chinese and Pakistani isolates.The study indicates that the pathogen causing the disease of Simmental cattle in a cattle farm in Kashgar is Thaleria annulata.
关键词
喀什/环形泰勒虫/PCR/18SrRNA/鉴定分析Key words
Kashgar/Thaleria annulata/PCR/18S rRNA/Identification and analysis引用本文复制引用
基金项目
国家自然科学基金资助项目(31960705)
国家自然科学基金资助项目(32160841)
塔里木大学研究生科研创新项目(TDGRI202240)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据