Pathogens isolation and identification of co-infection between Escherichia coli and Clostridium perfringens type A in sheep
The purpose of the study was to diagnose the death of suspected co-infection of Escherichia coli and Clostridium perfringens type A in a sheep farm in Ningxia and put forward corresponding prevention and control plans.Clostridium perfringens chromogenic medium and eosin blue solid medium were used for pathogen isolation and culture,and the isolated strains were detected by PCR with 16S rRNA gene sequence,and the molecular phylogenetic tree of the isolate was constructed based on the 16S rRNA sequence.After that,the toxin factors of Escherichia coli isolates were detected by PCR,and the toxin typing of Clostridium perfringens isolates was performed.The results showed that the PCR detection of isolates obtained 16S rRNA-specific bands.The results of molecular phylogenetic tree showed that the isolate strain of Escherichia coli was NXDC001 in the same branch as Escherichia coli,and the isolate strain of Clostridium perfringens was NXSJO01 in the same clade as Clostridium perfringens.Escherichia coli isolates detected toxin factors HPI(irp2)and LEE(eae).Clostridium perfringens isolates carried the toxin factor cpa,which proved that the isolate was Clostridium perfringens type A.The results showed that one strain of Escherichia coli and Clostridium perfringens type A were successfully isolated and identified,which proved that the dead sheep were co-infected with Escherichia coli and Clostridium perfringens type A.
Escherichia coliClostridium perfringens type ACo-infectionSheep
NETL
NSTL
万方数据
维普
