摘要
试验旨在了解河北省秦皇岛地区养殖场鸡源大肠杆菌耐药情况,为该地区鸡大肠杆菌病的用药方案提供参考.采集秦皇岛市部分地区鸡场疑似感染大肠杆菌的病死鸡病料,采用细菌分离培养、革兰氏染色法和PCR鉴定对分离株进行鉴定,并进行药敏试验.结果显示:共检测出18株大肠杆菌,分离株对庆大霉素、丁胺卡那敏感;对哌拉西林、头孢哌酮、羧苄西林和头孢曲松呈中度耐药,耐药率为33.33％～50.00％;对青霉素、头孢他啶、苯唑西林、红霉素、头孢拉定和头孢氨苄高度耐药,耐药率为94.44％～100.00％.研究表明,应不定时检测当地鸡源大肠杆菌对常用抗生素药物的敏感性,以便及时快速地调整用药,避免造成耐药性.
Abstract
The aim of the experiment was to understand the drug resistance of chicken Escherichia coli in Qinhuangdao area of Hebei Province,and to provide a reference for the drug scheme of chicken colibacillosis in the area.Samples of dead chickens suspected to be infected with Escherichia coli were collected from chicken farms in Qinhuangdao City.The isolates were identified by bacterial isolation culture,Gram staining,and PCR,and drug susceptibility test was carried out.The results showed that a total of 18 Escherichia coli strains were detected,and the isolates were sensitive to gentamicin and butanicana.It was moderately resistant to piperacillin,cefoperazone,carbenicillin,and ceftriaxone,with resistance rates ranging from 33.33％to 50.00％.It was highly resistant to penicillin,ceftazidime,oxacillin,erythromycin,cefradine,and cephalexin,and the resistance rate was 94.44％～100.00％.Studies have shown that the sensitivity of local chicken Escherichia coli to commonly used antibiotics should be detected from time to time in order to adjust drug use quickly and avoid drug resistance.
国家科技期刊平台
NSTL
维普
万方数据