Study on suspension culture technology of canine parvovirus
The aim of the study was to demonstrate the possibility of suspension culture of CPV,improve the viral content of CPV P6 strain antigen,reduce the quality difference of vaccine between different batches produced by rotating bottle,and produce vaccine products with stable quality.The high density culture of Vero cells was realized by utilizing the huge surface area provided by BioNOC type Ⅱ microcarrier of fiber braided fabric with large pores,and the preparation process of CPV P6 strain antigen was established in a Tide-cell bioreactor.The results show that 1.0 × 1010 F81 seed cells were added into the bioreactor Tide-cell carrier tank and cultured under optimized parameter conditions.After 96 h culture in RPMI 1640 medium,the total number of cells was 1.0 × 1011 and glucose consumption was about 5 g/(L·h).The production seed virus CPV P6 strain was added into the Tidecell microcarrier cell culture vial according to the MOI=0.02,and the RPMI 1640 virus maintenance solution containing 8％serum was added to 50 L,the pH value was adjusted to 7.2～7.3,and the culture was harvested for 48 h.The virus content should be at least 107.43 TCID50/mL.
NETL
NSTL
万方数据
维普
