研究旨在确诊广西某肉鸽场中出现神经症状的病鸽并为该场提供相应的防治方案,通过临床诊断、病理解剖、分子生物学检测、细菌分离、病毒分离等方法对广西某肉鸽场病鸽进行诊断.结果显示,剖检可见病鸽肝脾肿大、坏死,花斑肾;分子生物学检测结果显示,鸽Ⅰ型副黏病毒(pigeon paramyxovirus type 1,PPMV-1)、圆环病毒(pigeon circovirus,PiCV)和疱疹病毒(pigeon herpesvirus,PiHV)为阳性;对病样进行细菌培养,结果未分离到细菌;将病样接种SPF鸡胚进行病毒分离,结果显示,尿囊液血凝效价稳定在8log2,其血凝性可被新城疫病毒(NDV)阳性血清抑制,但不能被禽流感病毒(AIV)阳性血清抑制.研究表明,最终诊断结果为PPMV-1、PiCV和PiHV混合感染.
Diagnosis of pigeon paramyxovirus type 1,pigeon circovirus and pigeon herpesvirus co-infection
The aim of the study was to identify the sick pigeons with neurological symptoms in a meat pigeon farm in Guangxi and to provide corresponding prevention and control programs for the farm.The sick pigeons were diagnosed by clinical diagnosis,pathological anatomy,molecular biological detection,bacterial isolation,and virus isolation.The results showed that the liver and spleen were swollen,necrotic and mottled kidney.The results of molecular biological tests showed that PPMV-1,PiCV,and PiHV were positive.The samples were cultured with bacteria,and no bacteria were isolated.The results showed that the hemagglutination titer of allantoic fluid was stable at 8 log2,and its hemagglutination could be inhibited by NDV positive serum,but could not be inhibited by AIV positive serum.Studies have shown that the final diagnosis is a mixed infection of PPMV-1,PiCV,and PiHV.
Pigeon paramyxovirus type 1Pigeon circovirusPigeon herpesvirus
NETL
NSTL
万方数据
维普
