Cloning and genetic evolution analysis of Cap and Rep genes of pigeon circovirus in Guangxi
The purpose of the study was to understand the genetic variation of Cap and Rep genes of PiCV in a meat pigeon farm in Guangxi.Cap and Rep genes were cloned,sequenced and analyzed from pigeon tissue samples infected with PiCV by PCR.The results showed that the cloned Cap and Rep genes were 816 bp and 954 bp,respectively.The results of nucleotide sequence homology analysis showed that the cloned Cap and Rep genes had the highest nucleotide homology with XJ1 strain,which were 95.5%and 97.6%,respectively.Genetic evolution analysis showed that Cap and Rep genes were closely related to XJ1 strain.The results showed that PiCV was prevalent in Guangxi pigeon population,and its Cap and Rep gene had the highest nucleotide homology with XJ1 strain,which provided an important reference for the diagnosis and control of PiCV in Guangxi.
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万方数据
