Pathogen detection,isolation and identification of Clostridium perfringens in sheep in large-scale farms in southern Xinjiang
The purpose of this study was to understand the prevalence of Clostridium in sheep farms in southern Xinjiang.A total of 421 feces and silage samples from nine large-scale sheep farms in Bazhou,Aksu,and Hotan in southern Xinjiang were collected aseptically,and six tissue samples from three dead sheep suspected of Clostridium were collected.Through bacterial isolation and culture,Gram staining,biochemical identification,16S rRNA gene identification,bacterial typing and drug sensitivity test were carried out.The results showed that 71 strains of Clostridium perfringens were isolated from 427 samples,the total isolation rate was 16.63%,and 25 of 119 silage samples were positive,the detection rate was 21.01%(25/119).Among 302 sheep feces samples,40 were positive,and the detection rate was 13.24%(40/302).Six clinical samples were all positive,and the detection rate was 100.00%.Among 71 strains of Clostridium perfringens,57 strains of Clostridium perfringens type A,four strains of Clostridium perfringens type D and 10 strains of Clostridium perfringens type F.The results of drug sensitivity test showed that the isolates were highly sensitive to penicillin,and resistant to sulfamethoxazole,doxycycline,tetracycline,gentamicin and polymyxin B.The research shows that the infection risk of Clostridium perfringens in large-scale sheep farms in southern Xinjiang is high,which needs to be paid enough attention to in feeding and management.
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万方数据
