红景天提取物对动物皮肤保护作用的研究
Study on the protective effect of Rhodiola rosea L.extract on animal skin
叶佳滨 1赵俊钢 2张凯妮 1付梦丽 1王枳懿 3韩硕 4赵文刚1
作者信息
- 1. 温州大学,浙江 温州 325035
- 2. 重庆医科大学附属儿童医院,重庆 400014
- 3. 锦州医科大学医疗学院,辽宁 锦州 121000
- 4. 辽宁省农业发展中心,辽宁 沈阳 110032
- 折叠
摘要
试验旨在研究红景天提取物(RE)对动物皮肤的保护作用.试验采用紫外线(UVB)辐照诱导角质形成细胞(HaCaT)和小鼠背部皮肤分别建立动物皮肤体外、体内损伤模型.通过检测HaCaT细胞中的DNA损伤、活性氧、炎症因子表达水平和小鼠背部表皮厚度和DNA损伤评估RE对皮肤损伤的保护作用.结果显示,RE可以减少紫外线诱导的HaCaT细胞损伤模型中的DNA损伤,降低细胞中活性氧含量和炎症因子的mRNA表达水平,减少小鼠背部皮肤损伤模型中表皮的厚度和DNA损伤.研究表明,RE对皮肤损伤具有保护作用.
Abstract
The purpose of the experiment was to study the protective effect of RE on animal skin.UVB irradiation was used to induce keratinocytes(HaCaT)and mouse back skin to establish animal skin injury models in vitro and in vivo.The protective effect of RE on skin injury was evaluated by detecting the levels of DNA damage,reactive oxygen species,inflammatory factors in HaCaT cells,the thickness of mouse dorsal epidermis and DNA damage.The results showed that RE could reduce DNA damage in HaCaT cells induced by ultraviolet rays,reduce the content of reactive oxygen species and mRNA expression of inflammatory factors in cells,and reduce the thickness of epidermis and DNA damage in mouse back skin injury model.Studies have shown that RE has a protective effect on skin injury.
关键词
红景天提取物/动物皮肤病/紫外线辐照/皮肤损伤Key words
Rhodiola rosea L.extract/Animal skin diseases/Ultraviolet irradiation/Skin damage引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据