Effects of donor superovulation and recipient simultaneous estrus on the effect of autumn aging Liaoning cashmere goat embryo transfer
The experiment was conducted to explore the application of embryo transfer technology to improve the efficiency of genetic resources utilization of high-quality aged Liaoning cashmere goat ewe.In autumn,eight high-quality Liaoning cashmere goat ewes with an average age of 6.75 years old and 28 Liaoning cashmere goat and dairy goat cross ewes with an average age of 6.64 years old were used as recipients,and to compare the effects of different superovulation and estrus synchronization methods on embryo transfer in aged cashmere goats.The results showed as follows:The estrus rate,average number of available embryos and availability of donor ewes in the superovulation group Ⅱ were significantly higher than those in the superovulation group Ⅰ(P<0.05).The estrus rate of recipient estrus in group Ⅰ was higher than that in group Ⅱ,but the difference was not significant(P>0.05).The pregnancy rate of recipient ewes in donor superovulation method Ⅱ+recipient simultaneous estrus method Ⅱ group was significantly higher than that in donor superovulation method Ⅰ+recipient simultaneous estrus method Ⅰ group(P<0.05),and the lambing rate was higher than that in donor superovulation method Ⅰ+recipient simultaneous estrus method Ⅰgroup,but the difference was not significant(P>0.05).According to the lambing situation of ewe receiving embryo transfer,the accuracy rate of early pregnancy diagnosis by B-ultrasound was 91.67％(11/12),and the detection rate of early pregnancy diagnosis by B-ultrasound was 88.89％(8/9).The results showed that the superovulation method Ⅱ group had the best effect on donor ewes,and the superovulation method Ⅱ+simultaneous estrus method Ⅱ group had the highest pregnancy rate and lambing rate,which was the best technique for embryo transfer of old Liaoning cashmere goat ewes.
Liaoning cashmere goatSuperovulationEmbryo transferEarly pregnancy diagnosis by B-ultrasoundPregnancy rate
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