以色列急性麻痹病毒LN株全基因克隆与遗传进化分析
Cloning and genetic evolution analysis of the whole gene of Israel acute paralysis virus LN strain
陈路 1李林熹 1梁爽 1韩宇 2李明 1孙莉1
作者信息
- 1. 锦州医科大学畜牧兽医学院,辽宁 锦州 121000
- 2. 锦州市农业农村综合服务中心(锦州市植物保护中心),辽宁 锦州 121000
- 折叠
摘要
研究旨在了解辽宁省以色列急性麻痹病毒(IAPV)的遗传进化特点.试验选取成年患病蜜蜂提取IAPV并进行PCR测序,选取China-BJ(GenBank登录号:KX421583.1)基因序列设计12对引物进行全基因克隆,将获得的基因序列与GenBank已公布的19株IAPV序列进行遗传进化分析.结果显示:试验成功分离出IAPV,并将其命名为IAPV-LN株(GenBank登录号:MZ269472.1).IAPV-LN株与China-BJ和China-BJ2(GenBank登录号:MG599488.1)同属一个分支.研究表明,丹东市某蜂场患病蜜蜂中分离得到的IAPV-LN株与China-BJ2、China-BJ的亲缘关系最近,可能来自同一个原始毒株.
Abstract
The purpose of the study was to understand the genetic and evolutionary characteristics of Israel acute paralysis virus(IAPV)in Liaoning Province.IAPV was extracted from adult diseased honeybees and sequenced by PCR.Twelve pairs of primers were designed to clone the whole gene of China-BJ(GenBank accession number:KX421583.1),and the obtained gene sequence was analyzed by genetic evolution with 19 IAPV sequences published by GenBank.The results showed that IAPV was successfully isolated and named IAPV-LN strain(GenBank accession number:MZ269472.1).IAPV-LN strain belongs to the same branch as China-BJ and China-BJ2(GenBank accession number:MG599488.1).The results showed that the IAPV-LN strain isolated from diseased bees in a bee farm in Dandong had the closest genetic relationship with China-BJ2 and China-BJ,and might come from the same original strain.
关键词
以色列急性麻痹病毒/全基因克隆/遗传进化分析Key words
Israeli acute paralysis virus/Entire gene cloning/Phylogenetic analysis引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据