Cloning and genetic evolution analysis of the whole gene of Israel acute paralysis virus LN strain
The purpose of the study was to understand the genetic and evolutionary characteristics of Israel acute paralysis virus(IAPV)in Liaoning Province.IAPV was extracted from adult diseased honeybees and sequenced by PCR.Twelve pairs of primers were designed to clone the whole gene of China-BJ(GenBank accession number:KX421583.1),and the obtained gene sequence was analyzed by genetic evolution with 19 IAPV sequences published by GenBank.The results showed that IAPV was successfully isolated and named IAPV-LN strain(GenBank accession number:MZ269472.1).IAPV-LN strain belongs to the same branch as China-BJ and China-BJ2(GenBank accession number:MG599488.1).The results showed that the IAPV-LN strain isolated from diseased bees in a bee farm in Dandong had the closest genetic relationship with China-BJ2 and China-BJ,and might come from the same original strain.
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