Evaluation of disinfection effect of four disinfectants on common pathogenic bacteria in large-scale dairy farms
The purpose of this experiment was to explore the efficacy of common disinfectants in killing dominant pathogens on airborne environment and animal surface in large-scale dairy farms,and to provide a reference for further formulating scientific and reasonable disinfection technical specifications.In the study,the dominant pathogens(Staphylococcus aureus,Escherichia coli,and Clostridium perfringens)were used as indicator bacteria,and the killing effect of common disinfectants(compound glutaral solution,Huisheng-30,potassium bisulfate,and povidone iodine)on bacterial propagules was discussed by suspension qualitative and quantitative tests.The results showed that 1:100 times diluted compound glutaral solution disinfectant could effectively kill Staphylococcus aureus,Clostridium perfringens,and Escherichia coli after 1,20,5 min.The disinfectant solution diluted by 1:200 times could effectively kill Staphylococcus aureus,Clostridium perfringens,and Escherichia coli after 10,20,15 min treatment.1:200 times diluted potassium bisulfate disinfectant could effectively kill Staphylococcus aureus,Clostridium perfringens,and Escherichia coli after 3,5,5 min treatment.Povidone iodine disinfectant diluted 1:400 times could effectively kill Staphylococcus aureus,Clostridium perfringens,and Escherichia coli after treatment for 15,20,20 min.The results indicates that Staphylococcus aureus and Escherichia coli were sensitive to compound glutaral solution,while Clostridium perfringens was sensitive to potassium persulfate complex powder.The four disinfectants should be used alternately to avoid the emergence of drug-resistant strains.