The purpose of this study is to realize LMH adherent cell suspension culture,stable passage and high density growth,and apply it to serum avian adenovirus type 4(FAdV-4)suspension culture.In this study,LMH adherent cells were acclimated with low serum content,screened by serum-free suspension culture and blind transmission test of FAdV-4 RP-4 strain.The results showed that LMH suspension cells could be cultured in serum-free medium for the third generation,and the final cell density was over 4.00×106 cells/mL.The toxicity of FAdV-4 could reach 108.13 TCID50/0.1 mL when cultured to the fourth generation.The results showed that LMH adherent cells could be domesticated in suspension with specific medium,and could be applied to the stable proliferation of FAdV-4.
LMH adherent cellsDomesticationSuspension cultureAvian adenovirus type 4(FAdV-4)
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