LMH贴壁细胞悬浮驯化及病毒增殖研究
Study on suspension domestication of LMH adherent cells and virus proliferation
任培森 1吕文静 2黄金海 3刘海霞 2蔡天宁2
作者信息
- 1. 天津大学生命科学学院,天津 300072;天津瑞普生物技术股份有限公司,天津 300381
- 2. 天津瑞普生物技术股份有限公司,天津 300381
- 3. 天津大学生命科学学院,天津 300072
- 折叠
摘要
研究旨在实现LMH贴壁细胞悬浮培养、稳定传代、高密度生长,并应用于血清4型禽腺病毒(FAdV-4)悬浮培养.研究进行LMH贴壁细胞低含量血清适应驯化,并进行无血清悬浮培养筛选及FAdV-4 RP-4 毒株接毒盲传试验.结果显示:LMH悬浮细胞可在无血清培养基中传代第 3 代后,最终细胞密度达4.00×106 cells/mL以上;FAdV-4连续培养至第4代,毒价可达108.13TCID50/0.1 mL.研究表明,用特定培养基可使LMH贴壁细胞实现悬浮驯化,并可应用于FAdV-4稳定增殖.
Abstract
The purpose of this study is to realize LMH adherent cell suspension culture,stable passage and high density growth,and apply it to serum avian adenovirus type 4(FAdV-4)suspension culture.In this study,LMH adherent cells were acclimated with low serum content,screened by serum-free suspension culture and blind transmission test of FAdV-4 RP-4 strain.The results showed that LMH suspension cells could be cultured in serum-free medium for the third generation,and the final cell density was over 4.00×106 cells/mL.The toxicity of FAdV-4 could reach 108.13 TCID50/0.1 mL when cultured to the fourth generation.The results showed that LMH adherent cells could be domesticated in suspension with specific medium,and could be applied to the stable proliferation of FAdV-4.
关键词
LMH贴壁细胞/驯化/悬浮培养/血清4型禽腺病毒(FAdV-4)Key words
LMH adherent cells/Domestication/Suspension culture/Avian adenovirus type 4(FAdV-4)引用本文复制引用
基金项目
天津市智能制造专项项目(21ZNCXSN00020)
天津市科技支撑重点项目(22YFZCSN00080)
出版年
2024
国家科技期刊平台
NSTL
万方数据