Study on suspension domestication of LMH adherent cells and virus proliferation
The purpose of this study is to realize LMH adherent cell suspension culture,stable passage and high density growth,and apply it to serum avian adenovirus type 4(FAdV-4)suspension culture.In this study,LMH adherent cells were acclimated with low serum content,screened by serum-free suspension culture and blind transmission test of FAdV-4 RP-4 strain.The results showed that LMH suspension cells could be cultured in serum-free medium for the third generation,and the final cell density was over 4.00×106 cells/mL.The toxicity of FAdV-4 could reach 108.13 TCID50/0.1 mL when cultured to the fourth generation.The results showed that LMH adherent cells could be domesticated in suspension with specific medium,and could be applied to the stable proliferation of FAdV-4.
LMH adherent cellsDomesticationSuspension cultureAvian adenovirus type 4(FAdV-4)
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