Isolation,identification,and drug resistance analysis of Pasteurella multocida Liaoning strain from deer source
The study aimed to confirm the serotype and drug resistance characteristics of Pasteurella multocida from deer source.Sterile collection of diseased tissues such as heart,liver,lung,and spleen from the sick deer in a deer farm was conducted for bacterial isolation,culture,molecular biological identification,biochemical tests,pathogenicity tests,and drug sensitivity tests.The results showed that one strain of Pasteurella multocida from deer source was identified through bacteriological and molecular biological methods.The isolated strain formed round,smooth,translucent colonies on 10%sheep blood agar medium,and Wright's staining revealed small coccobacilli with polar intensification.The serotype-specific primer identification method confirmed the strain as capsular serotype D.Pathogenicity tests indicated that the infection rate of mice after infection with the isolated strain was 100%,with a mortality rate of 50%,and exhibited multiple organ hemorrhages and degenerative changes in the kidneys,liver,and intestinal mucosa.The isolates were sensitive to 12 drugs,including ampicillin,amoxicillin/clavulanic acid and meropenem.The study indicated that the Pasteurella multocida from deer source had strong pathogenicity and was still sensitive to most antibiotics,which may be related to the living environment of deer and few daily drugs.
DeerPasteurella multocidaIsolation and identificationPathogenicityDrug resistanceLiaoning strain
万方数据
维普
