Research on the preparation process of leonurine liposomes
The study aimed to optimize the preparation process of leonurine liposomes to achieve high encapsulation efficiency and small particle size.The trial evaluated the encapsulation efficiency,particle size,and polydispersity index(PDI)as indicators to select the most suitable method for preparing leonurine liposomes.Subsequently,the encapsulation efficiency was used as the evaluation criterion to compare the effects of the drug-to-lipid ratio,cholesterol-to-phospholipid ratio,PBS solution volume,ultrasonication time,and surfactant concentration on the encapsulation efficiency of leonurine liposomes.The more significant factors affecting the encapsulation efficiency were identified for response surface testing,and the optimal conditions obtained were verified in a confirmation test.The results showed that the thin-film dispersion method combined with the pH gradient method prepared leonurine liposomes with the best drug-to-lipid ratio of 1∶15.84,cholesterol-to-phospholipid ratio of 1∶13.4,and PBS solution volume of 9.92 mL.Under these conditions,the encapsulation efficiency of leonurine liposomes was 92.18%,the average particle size was 56.01 nm,and the PDI was 0.095.The study indicates that the thin-film dispersion method combined with the pH gradient method for preparing leonurine liposomes has a high encapsulation rate,meeting the trial requirements.
万方数据
维普
