Analysis of the gut microbiota of captive Trachypithecus francoisi based on 16S rRNA sequencing technology
The study aimed to clarify the structure and function of the gut microbiota of captive Trachypithecus francoisi.High-throughput 16S rRNA sequencing technology was used to analyze fecal samples from three adult individuals(male denoted as M,and females as Fa and Fb).The results showed that a total of 10 phyla were annotated among the three individuals,with Firmicutes and Bacteroidetes being the dominant bacterial phyla.The relative abundances of Firmicutes and Spirochaetes in male individual M were significantly lower than those in females Fa and Fb(P<0.05),and the relative abundance of WPS-2 was extremely higher in M than in Fa and Fb(P<0.01).A total of 76 genera were annotated among the three individuals.The relative abundance of the unclassified family Lachnospiraceae and f_Ruminococcaceae_unclassified in male individual M was significantly higher than in Fa and Fb(P<0.05),and the relative abundances of f_uncultured_rumen_bacterium_unclassified were extremely higher in M than in Fa and Fb(P<0.01).The relative abundances of Ruminococcus UCG-005 and Treponema_2 in M were significantly lower than in Fa and Fb(P<0.05),and the relative abundances of Ruminococcus UCG-002,Ruminococcus UCG-013,f_Ruminococcaceae_unclassified,and Ruminococcus UCG-014 were extremely lower in M than in Fa and Fb(P<0.01).The dgA-11_gut_group was not found in M,and Fusobacterium was not found in Fa and Fb.KEGG analysis revealed that the relative abundance of metabolic pathway-enriched genes in M was extremely higher than in Fa and Fb(P<0.01),but the relative abundance of cell process pathway-enriched genes was extremely lower than in Fa and Fb(P<0.01).The study indicates that there are significant differences in the composition and function of the gut microbiota between male and female captive Trachypithecus francoisi,which may be mainly related to physiological differences between sexes.
万方数据
维普
