Research progress on molecular detection methods of swine acute diarrhea syndrome coronavirus
SADS-CoV is an emerging porcine alphacoronavirus that causes severe vomiting,diarrhea,and dehydration in piglets under 5 days old,leading to high mortality rates.Early and accurate diagnosis is of great practical significance for the scientific prevention and control of SADS-CoV infection.This article reviews the research progress on molecular detection methods for SADS-CoV,including ELISA,RT-PCR and its derivative techniques(multiplex RT-PCR,real-time fluorescent quantitative RT-PCR,multiplex real-time fluorescent quantitative RT-PCR,droplet digital PCR),and nucleic acid isothermal amplification technologies(LAMP,RPA).The advantages and disadvantages of these detection methods are summarized and discussed,and the future development direction of SADS-CoV molecular detection methods is prospected,aiming to provide a reference for the scientific prevention and control of SADS-CoV.
万方数据
维普
