Detection of antibiotic resistance genes in a bovine diarrhea Escherichia coli strain
The experiment aimed to analyze the reasons for antimicrobial resistance in Escherichia coli causing diarrhea in cattle and to determine the carriage of 16S rRNA methyltransferase genes.The susceptibility of the diarrhea-causing Escherichia coli to antimicrobial agents was tested using antibiotic sensitivity test strips,and the carriage of 16S rRNA methyltransferase genes in the isolated strains was detected by PCR.Additionally,Kunming white mice were injected intraperitoneally with diluted bacterial solution to observe the onset and death from the infection.The results showed that the Escherichia coli strain carried the rmtB resistance gene and was sensitive to ceftazidime,cefotaxime,colistin,and cefoperazone,but resistant to other eight types of antibiotics.The test mice began to show symptoms five hours after injection with the diluted bacterial solution and all died within 48 hours.The study indicates that this bovine diarrhea Escherichia coli strain is resistant to aminoglycoside drugs such as amikacin and kanamycin,suggests the need for rational and standardized use of antibiotics.
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