和田羊血清中生殖激素含量与产羔率的相关性研究
Correlation between reproductive hormone content in serum and lambing rate of Hotan sheep
王鑫昆 1黄巧艳 1李伟 1邢凤1
作者信息
- 1. 塔里木大学动物科学与技术学院,新疆 阿拉尔 843300;新疆生产建设兵团塔里木畜牧科技重点实验室,新疆 阿拉尔 843300
- 折叠
摘要
试验旨在探究不同产羔率的和田羊血清中5种生殖激素[促卵泡激素(FSH)、促黄体素(LH)、孕酮(P4)、睾酮(T)、雌二醇(E2)]的变化规律.试验通过观察和记录产羔率选取产单羔和多羔和田羊各25只,利用放射免疫分析法(RIA)测定血清中FSH、LH、T的水平,酶联免疫吸附法(ELISA)测定血清中E2和P4的水平,并对5个血清激素指标进行相关性分析.结果显示,多羔和田羊血清中FSH、P4水平显著高于单羔和田羊(P<0.05),LH水平极显著低于单羔和田羊(P<0.01).单羔和多羔和田羊血清中E2 和T的水平差异均不显著(P>0.05).相关性分析显示,在单羔和田羊血清中,FSH与LH呈极显著正相关(P<0.01),LH与E2呈显著正相关(P<0.05),P4与T呈极显著负相关(P<0.01).多羔和田羊血清中,FSH与LH呈显著正相关(P<0.05).研究表明,高水平的FSH、P4可能与和田羊多羔性状相关,FSH水平增加、LH水平降低也可能是和田羊产多羔的原因.
Abstract
The experiment aimed to explore the patterns of change in five reproductive hormones[follicle-stimulating hormone(FSH),luteinizing hormone(LH),progesterone(P4),testosterone(T),and estradiol(E2)]in the serum of Hotan sheep with different lambing rates.The experiment observed and recorded 25 single-lamb and multiple-lamb Hotan sheep,and used radioimmunoassay(RIA)to measure the levels of FSH,LH,and T in the serum,and enzyme-linked immunosorbent assay(ELISA)to measure the levels of E2 and P4 in the serum,and conducted a correlation analysis of the five serum hormone indicators.The results showed that the levels of FSH and P4 in the serum of multiple-lamb Hotan sheep were significantly higher than those of single-lamb Hotan sheep(P<0.05),and the level of LH was extremely lower than that of single-lamb Hotan sheep(P<0.01).There was no significant difference in the levels of E2 and T in the serum between single-lamb and multiple-lamb Hotan sheep(P>0.05).Correlation analysis showed that in the serum of single-lamb Hotan sheep,FSH was extremely positively correlated with LH(P<0.01),LH was significantly positively correlated with E2(P<0.05),and P4 was extremely negatively correlated with T(P<0.01).In the serum of multiple-lamb Hotan sheep,FSH was significantly positively correlated with LH(P<0.05).The study indicates that high levels of FSH and P4 may be associated with the multiple-lamb trait of Hotan sheep,and the increase in FSH levels and decrease in LH levels may also be the reason for the multiple lambing of Hotan sheep.
关键词
和田羊/产羔率/生殖激素/相关性分析Key words
Hotan sheep/Lambing rate/Reproductive hormone/Correlation引用本文复制引用
基金项目
兵团"强南"科技创新人才项目(2022CB00104)
出版年
2024
国家科技期刊平台
NSTL
万方数据