Co-solvent combined with organic acid pretreatment to improve the enzymatic hydrolysis and fermentation performance of lignocellulose
The experiment aimed to establish an acidic organic solvent pretreatment method by comparing the separation effects of corn straw components and the recovery of cellulose in different solvent/water systems under the catalytic action of various organic acids.The pretreated cellulose-rich solid obtained was subjected to enzymatic hydrolysis and simultaneous saccharification and fermentation to evaluate the impact of this pretreatment method on subsequent enzymatic hydrolysis and fermentation.The results showed that in an acetone/water solvent system(50wt%)with oxalic acid as a catalyst at 1wt%,a pretreatment at 150℃for 30 minutes could remove 91.5%of hemicellulose and 77.0%of lignin,with a cellulose retention rate of 88.7%.The recovered corn straw,after enzymatic hydrolysis,achieved a cellulose conversion rate of 84.5%,which is a 62.4%increase compared to untreated corn straw.The study indicates that the solvent/water pretreatment reaction system with organic acid as a catalyst effectively improved the enzymatic hydrolysis and fermentation performance of corn straw,providing a reference for the high-value utilization of lignocellulosic materials.
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