Analysis of high-altitude hypoxia adaptation in the 10th exon of the HIF-1α gene in Xinjiang sheep and Ovis ammon
The aim of this experiment was to explore the mechanism of hypoxia adaptation in Pamir sheep and whether there is convergent evolution in other animals such as Tibetan sheep,in order to provide molecular markers for breeding new Tashkurgan sheep lines adapted to the low oxygen environment in Pamir Plateau.Using Duolang sheep in Xinjiang Plain(Kashgar)as the control,samples of Tashkurgan sheep and Marco Polo Argali were collected.PCR was used to amplify the target gene,and agarose gel electrophoresis was used to detect the amplification products,sequencing and single nucleotide polymorphism(SNP)site analysis.The results showed that there were four polymorphic loci and five haplotypes in exon 10 of HIF-1α gene in 46 Duolang sheep,18 Tashkurgan sheep and 7 Ovis ammon,among which Hap2 and Hap3 were the main haplotypes,located in the genes of Ovis ammon and Tashkurgan sheep,respectively.The results showed that the polymorphism of polymorphic loci of Tashkurgan sheep and Ovis ammon was related to their adaptation to low oxygen environment in the plateau,and the frequency of mutant allele(A)of G39210A was positively correlated with the altitude trend,so it was presumed that AA genotype was related to low oxygen adaptation.
万方数据
维普
