Effects of Lactobacillus acidophilus and Astragalus polysaccharide on intestinal flora,diarrhea rate and immune function of white yak calves
This study was conducted to investigate the effects of Lactobacillus acidophilus and Astragalus polysaccharide on intestinal flora,diarrhea rate and immune function of white yak calves.Eighty(30±3)d male white yak calves with similar body condition were randomly divided into four groups with four replicates in each group and five calves in each replicate.All groups were fed alfalfa hay and starter food freely.The calves in the control group were fed milk replacer with appropriate temperature.The milk replacer in the groups Ⅰ,Ⅱ and Ⅲ was added with 1%Lactobacillus acidophilus,1%Astragalus polysaccharide,and 1%Lactobacillus acidophilus+1%Astragalus polysaccharide,respectively.The pre-feeding period lasted for 15 days,and the formal experiment period lasted for 45 days.The results showed as follows:On the 45th day,the number of fecal lactic acid bacteria in the groups Ⅰ,Ⅱ and Ⅲ was increased by 32.86%,12.58%and 48.11%compared with the control group(P<0.05),respectively,and the number of E.coli was decreased by 37.35%,25.59%and 53.24%(P<0.05),respectively.Compared with the control group,the diarrhea rate of calves in the groups Ⅰ,Ⅱ and Ⅲ was reduced by 44.99%,26.82%and 61.95%,respectively,and the duration of diarrhea was decreased by 36.17%,28.51%and 42.55%,respectively.Compared with the control group,the serum immunoglobulin G(IgG)content of calves in the group Ⅲ was increased by 19.50%(P<0.05),and that of calves in the groups Ⅰ and Ⅱ was increased by 6.74%and 4.61%(P>0.05).Serum immunoglobulin A(IgA)and immunoglobulin M(IgM)contents of calves in experimental groups were higher than those in control group,but the differences were not significant(P>0.05).The results showed that the addition of Lactobacillus acidophilus and Astragalus polysaccharide in milk replacer powder could effectively regulate the intestinal flora balance of white yak calves,significantly reduce the diarrhea rate of calves,and increase the serum immunoglobulin content.
Lactobacillus acidophilusAstragalus polysaccharideWhite yakCalfIntestinal floraDiarrhea rateImmune function