Isolation,identification,virulence and pathogenicity of Staphylococcus hyicus in a large-scale breeding farm
Exudative dermatitis occurred in piglets aged 10 to 40 days in a large-scale farm,and the cause and pathogen were determined by laboratory diagnosis.The skin tissue of infected piglets was collected for bacterial isolation,and the pathogenic bacteria were determined by mouse lethal test and piglet virulence test.The pathogenic bacteria were observed by morphology,analyzed by biochemical characteristics,detected by virulence gene and analyzed by drug susceptibility.The results showed that one isolated strain was obtained after isolation and purification,and its morphological and biochemical characteristics were consistent with that of Staphylococcus hyicus.It was named JS strain.All the mice infected by the isolated strain could be killed by peritoneal infection at the dose of 109 CFU/mL,and the piglets infected subcutaneously at the dose of 108 CFU/mL could reproduce exudative dermatitis cases.Staphylococcal epidermidin Exh A and Shet A were positive,while Exh B,Exh C,Exh D,and Shet B were negative.The results of drug sensitivity test showed that the isolated strain were sensitive to cefuroxime,moderately sensitive to cefazolin,ceftriaxone and cefalexin,and showed varying degrees of resistance to 14 kinds of antibiotics such as cefoperazone and cephaloadine.The study showed that the pathogen causing piglet exudative dermatitis in this farm was Staphylococcus hyicus,and the isolates carried two virulence genes and showed multiple drug resistance.
Staphylococcus hyicusExudative epidermitisIsolation and identificationDrug resistancepathogenicity
万方数据
维普
