Analysis of antibiotic resistance phenotype and genotype of Escherichia coli from pets in Shihezi,Xinjiang
In order to understand the status of drug resistance and drug resistance gene carrying of pet-derived Escherichia coli in Shihezi city,56 anal swabs from dogs and cats were collected for isolation and culture of Escherichia coli.The minimum inhibitory concentration of the isolates against 16 commonly used antibiotics was determined by microbroth dilution method,and the carrying status of drug resistance genes was detected by PCR.The results showed that a total of 50 Escherichia coli isolates were isolated(89.29%),among which 50 isolates were the most resistant to ampicillin(52.0%).There were different degree of resistance to 10 kinds of drugs,such as ofloxacin,and the resistance rate was 10.0%～38.0%.The results of drug resistance gene detection showed that the detection rates of tetracycline(tetK,tetM),β-lactams(blaCTX-M,blaFOX)and sulfonamides(sul2)were all above 30.0%.The results show that multiple drug resistance is common in pet-derived Escherichia coli in Shihezi.Due to the complex generation and transmission mechanism of drug resistance genes,the transmission risk needs to be further explored and evaluated.
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