Effect of intraperitoneal injection of aminoxyacetic acid on spontaneous colitis of IL-10-/-aged rats
Objective To explore the intervention effect of aminoxyacetic acid(AOAA)on IL-10-/-aged mice.Methods Male 6-8-week-old IL-10-/-mice were fed to 15 weeks to establish the model of spontaneous colitis.The mice were randomly divided into CTRL group(n = 5)and AOAA group(n = 5).The mice in the AOAA group were injected in-traperitoneally with AOAA 5 mg/(kg·d),while the mice in CTRL group were given the same volume of sterile PBS for 40 days.The fecal characteristics and anal blood of mice were observed every day,the length of colon and spleen index were calculated,the levels of IL-6 and IL-13 in colon tissue were determined by ELISA method,and the mononuclear cells in the lamina propria of colon were taken for flow cytometry analysis.Results Compared with CTRL group,the symptoms and signs of colitis in AOAA group were relieved.Compared with CTRL group,the colon length of AOAA group was lon-ger,the spleen index was higher,the levels of IL-6 and IL-13 in colon tissue were decreased,the percentage of macro-phages and monocytes in the lamina propria of colon mucosa was decreased,the percentage of CD80+ cells(M1 macro-phage marker)in the lamina propria was decreased,the percentage of CD206+ cells(M2 macrophage marker)in the lami-na propria was higher(all P<0.01).Conclusion AOAA has a protective effect on senile colitis induced by IL-10 defi-ciency,which may be related to balancing the polarization of macrophages and regulating the secretion of inflammatory cy-tokines.
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