A study on the relationship between the level of plasma trimethylamine N-oxide and age and gender in the healthy aging population
Objective To explore the factors affecting the level of plasma trimethylamine N-oxide(TMAO)during healthy aging.Methods Random number was used to sample healthy individuals who underwent physical examinations at Community Hospitals in Baoshan District from December 2020 to January 2021.A total of 87 subjects were selected and in-formed consent was signed.Their age distribution ranged from30 to96 years old,with an average age of(69.3±17.4)years,including 39 males and 48 females.Their clinical data were collected by means of community electronic medical records and their glomerular filtration rate(eGFR)was estimated according to CKD-EPI.Ultra-high performance liquid chromatography-mass spectrometry(UHPLC-MS)was used to detect absolute quantification of plasma TMAO and its precursor trimethylamine(TMA)of these subjects.The clinical data,TMAO and TMA were compared among groups based on gender.Factors associ-ated with age and TMAO were analyzed.Partial correlation analysis was used to investigate the factors affecting plasma TMAO content during aging process.Results There were gender differences in creatinine,red blood cell count,hemoglobin,plate-lets,uric acid,direct bilirubin,and total cholesterol(P＜0.05).Blood urea nitrogen,TMAO,monocyte count,total bile acid and creatinine were significantly positively correlated with age(P＜0.05),while red blood cells,hemoglobin,platelets,glucose,total protein,albumin,total cholesterol,alanine aminotransferase,calcium,and kidney filtration rate were signifi-cantly negatively correlated with age(P＜0.05).Age,monocytes,creatinine,total bile acid,blood urea nitrogen,choline were significantly positively correlated with TMAO,while eGFR was significantly negatively correlated with TMAO(P＜0.05).After adjusting for the effects of TMA and eGFR on TMAO,plasma TMAO content was still significantly positively correlated with age(P＜0.05).Conclusion Plasma TMAO levels increase significantly during the healthy aging process.Even after ad-justing for its precursor sources(TMA)and elimination(eGFR),TMAO still exhibits a significant positive correlation with age,indicating that aging is an independent factor for the increase of plasma TMAO level.
国家科技期刊平台
NSTL
万方数据
维普
