Mechanism of PM2.5 induced apoptosis of vascular endothelial cells in elderly patients with coronary heart disease
Objective To analyze the correlation between the cardiac function grading of elderly patients with coronary heart disease(CHD)and the concentration of fine particulate matter(PM2.5)in the atmosphere of this city,explore whether PM2.5 can induce apoptosis of vascular endothelial cells by cultured vascular endothelial cells in vitro,and further explore the possible roles and mechanisms of inflammation and oxidative stress in it.Methods This study included two parts.In the first part,103 elderly patients diagnosed with CHD in Wuhu Hospital of Traditional Chinese Medicine Affiliated to Anhui College of Traditional Chinese Medicine from January 2021 to September 2022 were retrospectively collected.According to their heart function grading,they were divided into 1～4 grading.The heart function grading of elderly CHD patients and the trend of change in PM2.5 concentration in the city's atmosphere were analyzed.First,the differences in PM2.5 concentration,blood pres-sure,diabetes history,glycosylated hemoglobin and hospitalization history were compared between the two groups of patients with＜3 grading and≥3 grading of heart function according to the cardiac function grading.Secondly,the differences in cardi-ac function grading,blood pressure,diabetes history,glycosylated hemoglobin and hospitalization history were compared be-tween the PM2.5＜75 μg/m3 group and PM2.5≥75 μg/m3 group according to PM2.5 concentration,respectively.In the second part,human umbilical vein endothelial cells(HUVECs)were cultured.After 48 hours of intervention with different concentra-tion gradients of PM2.5 on HUVECs,Tunel staining was used to detect cell apoptosis,fluorescence microscopy was used to ob-serve the expression and distribution of ROS,ELISA method was used to detect SOD activity,and RT-PCR method was used to detect the mRNA expression of inflammatory factor IL-6 in the cells.Results The results of the first part of this study showed that the cardiac function grading of elderly CHD patients was consistent with the trend of changes in PM2.5 concentration in the air of the local area.There was no statistically significant difference(P＞0.05)in the exposure of atmospheric PM2.5 concen-trationbetweenthetwogroupswithheartfunction＜3 grading and heart function≥3 grading(P＜0.05).The results of the second part of this study showed that different concentrations of PM2.5 could induce apoptosis of HUVECs.PM2.5 induced the increase of ROS expression and distribution in HUVECs in a concentration-dependent manner,and inhibited SOD activity in HUVECs(P＜0.05).Different concentrations of PM2.5 could significantly up-regulate the mRNA expression of inflammatory factor IL-6 in HUVECs(P＜0.05).Conclusion The severity of CHD in the elderly is related to PM2.5 concentration in the atmosphere.PM2.5 can induce apoptosis of vascular endothelial cells,and stimulate the expression of inflammatory factors and increase the level of oxidative stress,which may be one of the important mechanisms involved in the progression of CHD in eld-erly patients.
国家科技期刊平台
NSTL
万方数据
