Expression and role of apolipoprotein A1 in non-alcoholic steatohepatitis-related chronic kidney injury
Objective To explore the expression and role of apolipoprotein A1(apoA1)in non-alcoholic steatohepatitis(NASH)-related chronic kidney injury(CKD).Methods A NASH model was established by intraperitoneal injection of western high-calorie diet combined with low-dose carbon tetrachloride(CCl4).The normal diet and intraperitoneal injection of CCl4 alone were used as the controls.The renal function,histopathological changes,serum apoA1 level and expression level of apoA1 in kidney were detected.Results Compared with the normal diet group and the control group treated with CCl4 injec-tion alone,NASH mice showed increased serum creatinine,vacuolar degeneration of renal tubular epithelial cells,increased ex-pression of renal tubular injury markers NAG and Kim-1,and increased expression of renal tubular epithelial transdifferentiation markers α-SMA.The expression of apoA1 increased in renal tissue.It was expressed in the cytoplasm of proximal renal tubule epithelial cells,and concentrated on the brush margin.Immunohistochemical staining of continuous slice showed that the ex-pression site of apoA1 was highly consistent with that of NAG.However,there was no significant difference in serum apoA1 level between NASH mice and the control group.Conclusion In NASH-related chronic kidney disease,apoA1 is highly ex-pressed in proximal renal tubular epithelial cells,consistent with the site of renal injury,but not related to serum apoA1 level.The specific mechanism of action of apoA1 in NASH-related chronic kidney injury needs further in-depth research.
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